Moving beyond
stalled wounds

Cell viability is important in wound healing

In stalled wounds, cells are unresponsive, growth factor signaling is abnormal, and ECM production is impaired.1-6 For wounds in a non-healing trajectory, an amniotic membrane that contains viable cells may be the right choice.7,8

Although all amniotic tissue products start from the same raw material, different preservation methods can impact the characteristics of the products considerably:

Dehydration and sterilization

Retains some growth factor content but alters the ECM structure and, in some products, may devitalize the endogenous living cells9-12


Preserves growth factor/cytokine content and structural integrity; however, cryopreservation techniques may cause the loss of cell viability9-12

The next way forward

See the novel hypothermic storage method that keeps Affinity fresh, or talk to an Organogenesis Tissue Regeneration Specialist about how Affinity can create an environment to support wound healing in your patients.

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  1. Barrientos S, et al. Wound Repair Regen. 2008;16(5):585-601.
  2. Kim BC, et al. J Cell Physiol. 2003;195(3):331-336.
  3. Vande Berg JS, et al. Surg Clin North Am. 2003;83(3):509-520.
  4. Wall IB, et al. J Invest Dermatol. 2008;128(10):2526-2540.
  5. Falanga V. Lancet. 2005;366(9498):1736-1743.
  6. Frykberg RG, et al. Adv Wound Care. 2015;4(9):560-582.
  7. Data on file. DR-0008. Organogenesis Inc.
  8. McQuilling JP, et al. Int Wound J. 2017;14(6):993-1005.
  9. Johnson A, et al. Ann Plast Surg. 2017;79(2):203-213.
  10. Brantley JN, et al. Adv Wound Care. 2015;4(9):545-559.
  11. Cooke M, et al. J Wound Care. 2014;23(10):465-474, 476.
  12. Abshier S. Podiatry Today. 2015;28(11):20-26.